The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". 7 8 To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. Epub 2021 Feb 9. Microporation applications for enhancing drug delivery. Carbohydrate Polymers 99, 199-207. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. (1999). Ann Surg Oncol. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. eCollection 2018 Feb. Molecules. Study of temperature influence. Carbohydrate Research, 160, 205-214. Biomater Res 24, 22 (2020). Guner, A.; Kibarer, G. (2001). This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Carbohydr Polym. ; Rjumtsev, E.I. Carbohydrate Polymers, 36, 319-326. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. On single point determination of intrinsic viscosity. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Intrinsic viscosity molecular weight relationship. 2016;47:15964. Colloid Polym. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. PMC Viswanath, D.S. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. Ed., 9(2), 85-90. Please enable it to take advantage of the complete set of features! The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. 113, 81-86. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. (2003). 2000;3(9):31826. Hindered diffusion of dextran and ficoll in microporous membranes. 2015;32(5):170413. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. NCI CPTC Antibody Characterization Program. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. 2008;28(5):64557. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Soc., 21, 1, 78-86. Unauthorized use of these marks is strictly prohibited. FOIA Drug Discov Today Technol. Die Makromolekulare Chemie, 148(1), 229-238. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. These studies were carried out at the Hisko (Gunpo, Korea). Springer Nature. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Physicochemical studies of oligodextran. J Appl Toxicol. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. (2013). Carbohydrate Research, 340, 811-826. Walkenhorst, S.; Olivier, R. (2003). DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Journal of American Chemical Society, 76 (20), 5041-5052. 2011;16(2):518. Biomaterials, 22, 1891-1898. (2001). WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. A solvent at this temperature is called a theta solvent [53]. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). sharing sensitive information, make sure youre on a federal Carbohydrate Polymers 87, 405-410. J Control Release. Carbohydrate Polymers, 78, 902-907. Synthesis and characterization of blue dextrans. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Harding, Stephen E. (2005). Skin barrier disruption by acetone: observations in a hairless mouse skin model. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. After 24h, the cumulative permeation of FITC-dextran M.W. ; Tsuchiya, H.M.; Rist, C.E. Chem. Pharm Res. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Carbohydrate Polymers 95 360-365. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Int J Food Sci Nutr. Biophysical Journal, 27, 177-181. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. All branched polysaccharide types could be described approximately by the hyperbranching theory. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. Dextran produced from Weissella cibaria JAG8 was purified and characterized. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Carbohydrate Polymers 88 1440-1444. The Mark-Houwink a constant is close to 0.5 in poor solvents. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Progr. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Appl Biochem Biotechnol. Journal of Applied Polymer Science, 13, 2031-2032. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. J Cosmet Laser Ther. 2003;10:33847. Morris, G.A. Unable to load your collection due to an error, Unable to load your delegates due to an error. Pathan IB, Setty CM. Guner, Ali (1999). The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. Nat Biotechnol. Bookshelf WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Google Scholar. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Eng. Keywords: European Polymer Journal, 42, 1086-1092. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t
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